| 產(chǎn)品編號(hào) | 產(chǎn)品名稱 | 產(chǎn)品包裝 | 產(chǎn)品價(jià)格 |
| AF1156 | Hsp70 Rabbit Monoclonal Antibody | 50μl | 709.00元 |
| 來(lái)源 | 用途 | 交叉反應(yīng)性 | 分子量 |
| Rabbit | WB, IF, IHC, ICC, FC | H, M, R | 70.0kDa |
WB, Western blot; IP, Immunoprecipitation; IF, Immunofluorescence; IHC, Immunohistochemistry; ICC, Immunocytochemistry; FC, Flow Cytometry; ELISA, Enzyme-linked Immunosorbent Assay; ChIP, Chromatin Immunoprecipitation Assay.
H, Human; M, Mouse; R, Rat; C, Chicken; Cw, Cow; Dg, Dog; Gp, Guinea pig; Hm, Hamster; Hr, Horse; Mk, Monkey; Pg, Pig; Rb, Rabbit; S, Sheep; Z, Zebrafish; All, all species expected.
配套提供了Western一抗稀釋液，可以用于Western檢測(cè)或其它適當(dāng)用途時(shí)的一抗稀釋。
建議抗體使用時(shí)的稀釋比例如下(實(shí)際使用時(shí)需根據(jù)抗原水平的高低作適當(dāng)調(diào)整)：
| WB | IP | IF | IHC | ICC | FC | ELISA | ChIP |
| 1:1,000-1:5,000 | - | 1:50-1:200 | 1:50-1:200 | 1:50-1:200 | 1:10-1:100 | - | - |
抗體詳細(xì)信息如下:
| About this Antibody |
| Name | Hsp70 Rabbit Monoclonal Antibody |
| Category | Rabbit Monoclonal Antibody (RabMAb); Primary antibody |
| Isotype | IgG |
| Purification | Affinity purification |
| About the Immunogen |
| Immunogen | Recombinant protein |
| Gene ID | 3303/3304(Human); 15511/193740(Mouse); 108348108/24472/294254(Rat) |
| SwissProt | P0DMV9/P0DMV8(Human); P17879/Q61696(Mouse); P0DMW0/P0DMW1(Rat) |
| Synonyms | HSP70-1B; HSP70-2 |
| Category | Cytoskeletal Signaling; Metabolism; Apoptosis |
| Background | The 70 kilodalton heat shock proteins (Hsp70s) are a family of conserved ubiquitously expressedheat shock proteins. Proteins with similar structure exist in virtually all living organisms. The Hsp70s are an important part of the cell's machinery for protein folding, and help to protect cells from stress. When not interacting with a substrate peptide, Hsp70 is usually in an ATP bound state. Hsp70 by itself is characterized by a very weak ATPase activity, such that spontaneous hydrolysis will not occur for many minutes. As newly synthesized proteins emerge from the ribosomes, the substrate binding domain of Hsp70 recognizes sequences of hydrophobic amino acid residues, and interacts with them. This spontaneous interaction is reversible, and in the ATP bound state Hsp70 may relatively freely bind and release peptides. However, the presence of a peptide in the binding domain stimulates the ATPase activity of Hsp70, increasing its normally slow rate of ATP hydrolysis.
|
